Research, Patents and Publications
Research Institutes and Education
University of South Florida, Tampa, FL
Proteomics, Metabolomics, and Mass Spec Core Facility Director
Northwestern University, Evanston, IL
Top-Down Proteomics, Instrument/Application Match, Histones, Cancer Oncology, + other
NHMFL, Tallahassee, FL
Managed Several Projects - From Environmental Analysis to OMICS to Intact Proteins
Scripps Florida, Jupiter, FL
Post Doc - Proteomics Core Facility
University of Florida, Gainesville, FL
Ph.D. in Bioanalytical Chemistry
Florida Institute of Technology, Melbourne, FL
B.S. in Research Chemistry
Public Works
Patents
2018
“Photoactivatable Probes and Uses Thereof”
Publication number: US10067136B1
Filed: February 6, 2017
Publication date: September 4, 2018
Applicants: University of South Florida
Inventors: Roman Manetsch, Arun Babu Kumar, Jeremiah Tipton
2014
“Target Binding Molecules Identified by Kinetic Target-Guided Synthesis”
Publication number: US20160116482A1
Filed: August 6, 2014
Publication date: April 28, 2016
Applicants: THE PENN STATE RESEARCH FOUNDATION, University of South Florida
Inventors: Roman Manetsch, Katya Pavlova Nacheva, David Lawrence Flanigan, Niranjan Kumar Namelikonda, Iredia David Iyamu, Sameer Shamrao Kulkarni, Megan M. Barber, Jeremiah Dwayne Tipton, Hong-Gang Wang, Kenichiro Doi
Publications
2023
Going beyond Binary: Rapid Identification of Protein–Protein Interaction Modulators Using a Multifragment Kinetic Target-Guided Synthesis Approach
Katya Nacheva, Sameer S. Kulkarni, Mintesinot Kassu, David Flanigan, Andrii Monastyrskyi, Iredia D. Iyamu, Kenichiro Doi, Megan Barber, Niranjan Namelikonda, Jeremiah D. Tipton, Prakash Parvatkar, Hong-Gang Wang, and Roman Manetsch*
J. Med. Chem. 2023, 66, 7, 5196–5207
2016
“3-Trifluoromethyl-3-aryldiazirine photolabels with enhanced ambient light stability”
Kumar, A,B; Tipton, J.D.; Manetsch, R., Chem. Commun. (Camb). 52, 2729-2732 (2016)
2014
“Site-specific human histone H3 methylation stability: fast K4me3 turnover.”
Zheng, Y.; Tipton, J. D.; Thomas, P. M.; Kelleher, N. L. Sweet, S.M.M.
Proteomics 14, (19) 2190–2199, (2014)
2014
“γ-AApeptide-based small-molecule ligands that inhibit Aβ aggregation.”
Wu, H.; Li, Y.; Bai, G.; Niu, Y.; Qiao, Q.; Tipton, J.D.; Cao, C.; and Cai, J. Chem. Commun. (Camb). 50, 5206-5208 (2014)
2013
“Light-dependent phosphorylation of Bardet-Biedl syndrome 5 in photoreceptor cells modulates its interaction with arrestin1.”
Smith, T.S.; Spitzbarth, B.; Li J.; Dugger, D.R.; Stern-Schneider, G.; Sehn, E.; Bolch, S.N.; McDowell, J.H.; Tipton, J.; Wolfrum, U.; Smith, W.C.;
Cell Mol Life Sci. 70 (23) 4603 – 4616. (2013)
https://doi.org/10.1007/s00018-013-1403-4
2013
“Oncogene Induced Cellular Senescence Elicits an Anti-Warburg Effect”
Li, M.; Durbin, K.; Sweet, S.S., Tipton, J.D.; Zheng,Y. and Kelleher, N.L.
Proteomics. 13 (17) 2585 – 8596 (2013)
2012
“Total Kinetic Analysis Reveals How Combinatorial Methylation Patterns are Established on Lysines 27 and 36 of Histone H3”
Zheng, Y.; Sweet, S.M.M.; Popovic, R.; Martinez-Garcia, E.; Tipton, J.D.; Thomas, P.M.; Licht, J.D.; and Kelleher, N.L. PNAS, 109 (34) 13549 – 13554 (2012)
2012
”Nano-LC FTICR Tandem Mass Spectrometry for Top-Down Proteomics: Routine Baseline Unit Mass Resolution of Whole Cell Lysate Proteins up to 72 kDa
”Tipton, J.D.; Tran, J.C.; Catherman, A.D.; Ahlf, D.R.; Durbin, K.R.; Lee, J.E.; Kellie, J.F.; Kelleher, N.L.; Hendrickson, C.L.; and Marshall, A.G.
Analytical Chemistry, 84 (5), 2111–2117 (2012)
2012
“High-Throughput, Robust Analysis of the Yeast Proteome <50 kDa Using Molecular Weight-Based Separations and Top Down Mass spectrometry"
Kellie, J.F.; Catherman, A.D.; Durbin, K.R.; Tran, J.C.; Tipton, J.D.; Norris, J.L; Witkowski, C.; Thomas, P.M.; Kelleher, N.L.”,
Analytical Chemistry, 84 (1), 209–215, (2012)
2011
“Mapping Intact Protein Isoforms in Discovery Mode Using Top Down Proteomics”
Tran, J.C.; Zamdborg, L.; Lee, J.E.; Catherman, A.D.; Durbin, K.R.; Ahlf, D.R.; Tipton, J.D.; Vellaichamy, A.; Kellie, J.F.; Li, M.; Wu, C.; Sweet, S.M.; Early, B.P.; Siuti, N.; Compton, P.D.; Thomas, P.M.; Kelleher, N.L.
Nature 480, 254–258, (2011)
2011
“Analysis of Intact Protein Isoforms by Mass Spectrometry”
Tipton, J.D.; Tran, J.C.; Catherman, A.D.; Ahlf, D.R.; Durbin, K.R.; Kelleher, N.L. Journal of Biological Chemistry, 286 (29), 25451-25458, (2011)
2011
Valence Parity to Distinguish c and z● Ions from Electron Capture Dissociation/Electron Transfer Dissociation of Peptides: Effects of Isomers, Isobars, and Proteolysis Specificity” Mao,Y.; Tipton, J.D.; Blakney, G.T.; Hendrickson, C.L.; Marshall, A.G. “ Analytical Chemistry, 83 (20), 8024-8028 (2011)
https://doi.org/10.1021/ac201619t
2010
Chromatographic Reduction of Isobaric and Isomeric Complexity of Fulvic Acids to Enable Multistage Tandem Mass Spectral Characterization.
Capley, E. N.; Tipton, J. D.; Marshall, A. G.; Stenson, A. C.
Analytical chemistry, 82 (19), 8194-202, (2010)
2010
Computing H/D-Exchange Rates of Single Residues from Data of Proteolytic Fragments.” Althaus, E.; Canzar, S.; Ehrler, C.; Emmett, M.R.; Karrenbauer, A.; Marshall, A.G.; Meyer-Bäse, A.; Tipton, J.D.; Zhang, H.M.;
BMC Bioinformatics, 11 (11), 424, (2010)
2010
New Reagents for Enhanced Liquid Chromatographic Separation and Charging of Intact Protein Ions for Electrospray Ionization Mass Spectrometry
Valeja, S.G.; Tipton, J.D.; Emmett, M.R.; Marshall, A.G.
Analytical Chemistry, 82 (17), 7515-7519, (2010)
2010
Sites and Extent of Selenomethionine Incorporation into Recombinant Cas6 Protein by Top-down and Bottom-up Proteomics with 14.5 T Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Wang, X.; Tipton, J.D.; Emmett, M.R.; Marshall, A.G. Rapid Communications in Mass Spectrometry, 24 (16), 2386-2392, (2010)
2010
Conformational States of Human Purine Nucleoside Phosphorylase at Rest, at Work and with Transition State Analogues. Edwards, A.A.; Tipton, J.D.; Brenowitz, M.D.; Emmett, M.R. Marshall, A.G; Evans, G.B.; Tyler, P.C.; Schramm, V.L.
Biochemistry, 49 (9), 2058-2067, (2010)
2009
A Robust 2-Dimensional Separation for Top-Down Tandem Mass Spectrometry of the Low Mass Proteome. Lee, J.E.; Kellie, J.F.; Tran, J.C.; Tipton, J.D.; Catherman, A.C.; Thomas, H.M.; Ahlf, D.R.; Durbin, K.R.; Vellaichamy, A.; Ntai, I.; Marshall, A.G.; and Kelleher, N.L. Journal of the American Society for Mass Spectrometry, 20 (12), 2183-2191 (2009)
2009
Sequential Proteolysis and High-Field FTICR MS To Determine Disulfide Connectivity and 4-Maleimide TEMPO Spin-Label Location in L126C GM2 Activator Tipton, J.D.; Carter, J.D.; Mathias, J.D.; Emmett, M.R.; Fanucci, G.E. and Marshall, A.G., Protein. Analytical Chemistry, 81 (18), 7611-7617 (2009)
2008
Enhanced Digestion Efficiency, Peptide Ionization Efficiency, and Sequence Resolution for Protein Hydrogen/Deuterium Exchange Monitored by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Zhang, H.; Kazazic, S.; Schaub, T.M.; Tipton, J.D.; Emmett, M.R. and Marshall, A.G., Analytical Chemistry, 80 (23), 9034-9041 (2008)
2006
2-Hydroxymethylboronate as a reagent to Detect Carbohydrates: Application to the Analysis of the Formose Reaction
Ricardo, A.; Frye, F.; Carrigan, M.A.; Tipton, J.D.; Powell, D.H.; Benner, S.A. “2-
Journal of Organic Chemistry, 71, 9503-9505 (2006)
1998
Qualitative Analysis of Herbs by Gas, Chromatography/Mass Spectrometry (GC/MS). An Undergraduate Instrumental Analysis Laboratory Exercise
Tipton, J.; Barnicki, T.; Smith, E.T.
The Chemical Educator, 3 (3), 1-12 (1998)